Fig. 1

Screening of anti-inflammatory compound library for anti-ASFV activity. ASFV-infected Vero cells were incubated with each compound at the defined concentration and the inhibitory effect on virus-induced CPE was measured by MTT assay at 72 h post-infection. (A) Percentage of anti-inflammatory compounds in 297-member library belonging to different molecular classes and experimental steps. (B) Z’ score of each plate in the screen, which was calculated by using the minimum (negative control: DMSO) and maximum (positive control: apigenin) MTT signals. (C) Percentage inhibition of virus-induced CPE by tested compounds. Compounds that inhibited ASFV-induced CPE by ≥ 50% and did not damage cell monolayer were defined as positive hits. All experiments were conducted in triplicate and mean values are presented for clarity. (D) Five top-performing compounds selected for further mechanistic studies